Hippocampal firing fields anchored to a moving object predict homing direction during path-integration-based behavior

Homing based on path integration (H-PI) is a form of navigation in which an animal uses self-motion cues to keep track of its position and return to a starting point. Despite evidence for a role of the hippocampus in homing behavior, the hippocampal spatial representations associated with H-PI are largely unknown. Here we developed a homing task (AutoPI task) that required a mouse to find a randomly placed lever on an arena before returning to its home base. Recordings from the CA1 area in male mice showed that hippocampal neurons remap between random foraging and AutoPI task, between trials in light and dark conditions, and between search and homing behavior. During the AutoPI task, approximately 25% of the firing fields were anchored to the lever position. The activity of 24% of the cells with a lever-anchored field predicted the homing direction of the animal on each trial. Our results demonstrate that the activity of hippocampal neurons with object-anchored firing fields can predict..., Mice were connected to the data acquisition system (RHD2000-Series Amplifier Evaluation System, Intan Technologies, analog bandwidth 0.09–7603.77 Hz, sampling rate 20 kHz) via a lightweight cable. The recording was controlled using ktan software (https://github.com/kevin-allen/ktan). Kilosort2 (https://github.com/jamesjun/Kilosort2) was used for spike extraction and clustering. Automatically generated clusters were visually inspected and manually refined with Phy (https://github.com/cortex-lab/phy).
The quality of spike clusters was estimated from the spike-time autocorrelation. A refractory period ratio was calculated from the spike-time autocorrelation from 0 to 25 ms (bin size: 0.5). The mean number of spikes from 0 to 1.5 ms was divided by the maximum number of spikes in any bin between 5 and 25 ms. Any cluster with a refractory period ratio larger than 0.25 was discarded.
In experiments with electrophysiological recordings, the position of the mouse was estimated from the position ..., , # Hippocampal firing fields anchored to a moving object predict homing direction during path-integration-based behaviour

Raw data recorded in the behavioural experiment or the electrophysiological experiment in the manuscript, titled 'Hippocampal firing fields anchored to a moving object predict homing direction during path-integration-based behaviour'.

# Description of the data and file structure

Here, we included the raw data from 2 experiments. 13 mice were included in the behavioural experiment (directory autopi_behavior_2021). 9 mice were included in the electrophysiological experiment (directory autopi_ca1). There is one directory per mouse. Within a mouse directory, you find one directory per recording session.

# Autopi\_ca1 subdirectory and files:

Most data files are in their respective recording session directories. The file names have either the recording session name (e.g. mn9686-27102021-0106) as file basename (e.g. `mn9686-27102021-0106.par`). Some other files don't ha...

基于路径整合的归巢（Homing based on path integration, H-PI）是一种导航方式，动物通过自身运动线索追踪自身位置并返回起点。尽管有证据表明海马体在归巢行为中发挥作用，但与H-PI相关的海马体空间表征仍知之甚少。本研究开发了一种归巢任务（AutoPI任务），要求小鼠在返回其巢穴前，在竞技场中找到随机放置的杠杆。对雄性小鼠CA1区的记录显示，海马神经元在随机觅食与AutoPI任务之间、明暗条件下的试验之间、以及搜索与归巢行为之间发生重映射。在AutoPI任务期间，约25%的发放场锚定在杠杆位置。具有杠杆锚定发放场的细胞中，24%的细胞活动可预测动物在每次试验中的归巢方向。我们的结果表明，具有物体锚定发放场的海马神经元活动可预测……小鼠通过轻质电缆连接至数据采集系统（RHD2000系列放大器评估系统，Intan Technologies，模拟带宽0.09–7603.77 Hz，采样率20 kHz）。记录由ktan软件（https://github.com/kevin-allen/ktan）控制。Kilosort2（https://github.com/jamesjun/Kilosort2）用于 spike 提取和聚类。自动生成的聚类通过Phy（https://github.com/cortex-lab/phy）进行视觉检查和手动优化。

Spike聚类的质量通过spike时间自相关评估。不应期比率由0至25 ms（ bin大小：0.5）的spike时间自相关计算得出：将0至1.5 ms内的平均spike数量除以5至25 ms内任意bin中的最大spike数量。不应期比率大于0.25的聚类被舍弃。

在电生理记录实验中，小鼠的位置由位置……，# 锚定在移动物体上的海马发放场预测基于路径整合行为中的归巢方向

本手稿中行为实验或电生理实验记录的原始数据，标题为《锚定在移动物体上的海马发放场预测基于路径整合行为中的归巢方向》。

# 数据与文件结构描述

本研究包含两项实验的原始数据。13只小鼠参与行为实验（目录autopi_behavior_2021）。9只小鼠参与电生理实验（目录autopi_ca1）。每只小鼠对应一个目录。在小鼠目录内，每个记录会话对应一个目录。

# Autopi_ca1子目录与文件：

大多数数据文件位于各自的记录会话目录中。文件名以记录会话名称（例如mn9686-27102021-0106）作为文件基名（例如`mn9686-27102021-0106.par`）。其他一些文件不……