Characteristics of the primers used for SYBR green Q-PCR assays.

aThe letters F and R at the end of the primer name indicate forward and reverse orientations, respectively.bTheoretical melting temperature (Tm) calculated using the MWG Oligo Property Scan (MOPS).

a. 引物名称末尾的字母F与R分别代表正向（forward）与反向（reverse）方向。
b. 理论解链温度（theoretical melting temperature, Tm）采用MWG Oligo属性扫描工具（MWG Oligo Property Scan, MOPS）计算得到。