Genome assembly and annotation of Oplegnathus fasciatus

Here we report a chromosome-level reference genome of <i>O. fasciatus</i> (from the southern population in the subtropical region) using the PacBio single molecule sequencing technique (SMRT) and High-through chromosome conformation capture (Hi-C) technologies. The genome was assembled into 120 contigs with a total length of 732.95 Mb and a contig N50 length of 27.33 Mb. After chromosome-level scaffolding, 24 chromosomes with a total length of 723.22 Mb were constructed. Moreover, a total of 27,015 protein-coding genes and 5,880 ncRNAs were annotated in the reference genome.

本研究报道了利用PacBio单分子实时测序技术（SMRT）与高通量染色体构象捕获（Hi-C）技术组装获得的O. fasciatus（亚热带南部种群）染色体级参考基因组。该基因组经初始组装得到120条重叠群（contig），总长度为732.95 Mb，重叠群N50长度为27.33 Mb。经染色体级支架锚定后，共构建得到24条染色体，总长度为723.22 Mb。此外，该参考基因组中共注释得到27015个蛋白质编码基因与5880个非编码RNA（ncRNAs）。