Low H3K27me3 and DNA hypomethylation define poorly prognostic pediatric posterior fossa ependymomas

A subgroup of Posterior fossa ependymomas show reduced H3K27me3, global DNA hypomethylation, are more invasive, exhibit poor prognosis and epigenetically deregulated genes converge on radial glial factors, suggesting developing cerebellar radial glia as candidate cells-of-origin. Overall design: RNA-sequencingwas performed in 15 posterior fossa (PF) ependymoma samples. 11 samples show low H3K27me3 and designated PF_H3K27me3-ve; 4 samples show high H3K27me3 and designated PF_H3K27me3+ve

一部分颅后窝室管膜瘤（Posterior fossa ependymomas）表现出组蛋白H3赖氨酸27三甲基化（H3K27me3）水平降低与全基因组DNA低甲基化特征，侵袭性更强且预后不良，其表观遗传失调的基因汇聚于放射状胶质细胞相关因子，提示发育中小脑放射状胶质细胞为该类肿瘤的候选起源细胞。整体实验设计：对15例颅后窝室管膜瘤样本进行RNA测序，其中11例样本的H3K27me3表达水平较低，命名为PF_H3K27me3-ve；4例样本的H3K27me3表达水平较高，命名为PF_H3K27me3+ve。