Single-cell RNA-Sequencing data of peri-gastrulation embryoids. Single-cell RNA-Sequencing data of peri-gastrulation embryoids

We analyzed scRNA-seq data in human pluripotent stem cells derived peri-gastrulation trilaminar embryonic disc (PTED) human embryo models with trilaminar embryonic disc-, amnion- and yolk sac-like structures. Overall design: scRNA-seq was performed on dissociated cells from PTED embryoids at three timepoints (day 2, day 5, day 8) and endoderm differentiation promoted PTED embryoids on day 3 and day14.

本研究分析了源自人类多能干细胞的原肠运动前期三胚层胚盘（PTED）人类胚胎模型的单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）数据，该模型具备三胚层胚盘样、羊膜样及卵黄囊样结构。实验设计：对三个时间节点（第2天、第5天、第8天）的PTED类胚胎体的解离细胞进行scRNA-seq测序，同时对经内胚层分化诱导的PTED类胚胎体分别在第3天和第14天开展测序。