Comparative proteome analysis of hAT-MSCs obtained from HTBD-hAT-MSCs, PTX-hAT-MSCs and LTBD-hAT-MSCs.

HTBD: Stem cells isolated from human adipose tissue obtained from a patient with secondary hyperparathyroidism (High turnover bone disease). LTBD: Stem cells isolated from human adipose tissue obtained from a patient with adynamic bone disease (Low turnover bone disease). (A) MSCs were subjected to protein isolation and proteins were loaded onto pH 5 to pH 8 IPG strips for the first dimension and precast SDS-PAGE gels for the second dimension separation and stained with Colloidal Coomassie Blue for 24 hr after 24 hours of fixation. The gels are representative of three gels from each MSC type. (B) The 2DE gel showing the protein spots that were cut from the gels and were subjected to MALDI-TOF/TOF analysis for identification.

HTBD：从继发性甲状旁腺功能亢进（高转换型骨病）患者的人体脂肪组织中分离得到的干细胞。LTBD：从动力缺失型骨病（低转换型骨病）患者的人体脂肪组织中分离得到的干细胞。(A) 对间充质干细胞（Mesenchymal Stem Cells, MSCs）进行蛋白质提取，将蛋白上样至pH 5至pH 8的固相pH梯度（Immobilized pH Gradient, IPG）胶条进行第一向电泳，随后采用预制十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, SDS-PAGE）凝胶完成第二向分离；固定24小时后，用胶体考马斯亮蓝染色24小时。所示凝胶为每类MSC三次重复实验的代表性凝胶。(B) 本双向凝胶电泳（Two-Dimensional Electrophoresis, 2DE）凝胶中标注了从凝胶中切取的蛋白斑点，上述斑点经基质辅助激光解吸电离飞行时间串联质谱（Matrix-Assisted Laser Desorption/Ionization Time-of-Flight/Time-of-Flight, MALDI-TOF/TOF）分析以实现蛋白质鉴定。