Transcriptome profiling and weighted gene co-expression network analysis of early floral development in Aquilegia coerulea

The current experiment was designed to obtain a broad characterization of the genetic pathways acting in early Aquilegia coerulea floral meristem development. We conducted an in-depth transcriptome profiling of early floral development in A. coerulea at four finely dissected developmental stages, with eight biological replicates per stage. . The developmental window we sequenced sampled stages that started with a late phase of stamen initiation, covered the period of FM termination, and ended with the initial stage of morphogenesis of the floral organs. Four developmental stages during early floral meristem development; Eight biological replicates per developomental stage. developmental stage1: stamen primordia initiating developmental stage2: staminode primordia finish initiating developmental stage3: rims of carpel primordia elevated developmental stage4: apex of floral bud consumed by developing carpel primordia

本实验旨在全面解析蓝花耧斗菜（Aquilegia coerulea）早期花分生组织发育过程中发挥作用的遗传通路。我们针对蓝花耧斗菜的早期花发育过程，在四个精细划分的发育阶段开展了深度转录组谱分析，每个阶段设置8个生物学重复。本次测序覆盖的发育窗口涵盖雄蕊起始晚期、花分生组织（Floral Meristem, FM）终止阶段，直至花器官形态建成的初始阶段。
早期花分生组织发育共包含四个发育阶段，各阶段具体信息如下：
阶段1：雄蕊原基起始
阶段2：退化雄蕊原基完成起始
阶段3：心皮原基边缘隆起
阶段4：花芽顶端被发育中的心皮原基占据