Evaluation of cell proliferation and endometrial thickness of bitches in different periods of diestrus

ABSTRACT This study aimed to determine the histological features of the endometrium of bitches, as well as the cell proliferation at specific moments of diestrus, 10, 20, 30, 40, 50 and 60 days post ovulation, correlating the endometrial thickness with the uterine cell proliferation and the metabolic state (weight, blood glucose and plasma cholesterol) of the animals. Therefore, the right and left uterine horns of 26 clinically healthy bitches submitted to ovariohysterectomy were histologically analyzed 10, 20, 30, 40, 50 and 60 days post ovulation. The hematoxylin-eosin and AgNOR staining techniques were performed. All parameters were evaluated by ANOVA and post-hoc Tukey test (p

摘要：本研究旨在明确母犬子宫内膜的组织学特征，以及排卵后10、20、30、40、50、60天这一间情期特定时间点的细胞增殖情况，并将子宫内膜厚度与子宫细胞增殖以及受试动物的代谢状态（体重、血糖及血浆胆固醇水平）进行相关性分析。为此，本研究对26只接受卵巢子宫切除术的临床健康母犬，在其排卵后10、20、30、40、50及60天时的左右子宫角开展组织学分析。实验采用苏木精-伊红（hematoxylin-eosin）染色与AgNOR染色技术。所有观测参数均通过方差分析（ANOVA）与Tukey事后检验进行评估（p