Leptosphaeria maculans gene expression at the beginning of primary oilseed rape leaf infection

The transcriptome of Leptosphaeria maculans was analysed in mycelium and during oilseed rape leaf infection. The array probes were designed from gene models from the L. maculans whole genome annotation. One aim of this study was to verify the expression of the automatically annotated gene models in various conditions. Another goal was to monitor gene expression profiles during oilseed rape leaf infection and to highlight tissue-specific transcripts, e.g. in plant up-regulated transcripts for further analyses. We performed 3 hybridizatiosn (NimbleGen) with samples derived from oilseed rape infected leaves. Samples from infected oilseed rape leaves were harvested 3 days post infection. Three replicates each. All samples were labeled with Cy3.

本研究针对油菜黑胫病菌（Leptosphaeria maculans）在菌丝体（mycelium）及油菜（oilseed rape）叶片侵染过程中的转录组（transcriptome）开展了分析。实验所用的基因芯片探针（array probes）均由该菌全基因组注释（whole genome annotation）中的基因模型（gene models）设计而来。本研究的目标之一为验证自动注释的基因模型（automatically annotated gene models）在不同条件下的表达情况；另一目标则是监测油菜叶片侵染过程中的基因表达谱，筛选组织特异性转录本（例如在寄主植物中上调表达的转录本）以供后续分析。我们采用NimbleGen芯片完成了3组杂交实验，样本取自接种油菜的感病叶片，于接种后3天（3 days post infection）采集，每组设置3个生物学重复（replicates），所有样本均以Cy3荧光染料标记。