Roles of Runx2 in chromatin landscape in skeletal development [RNA-Seq]

The transcriptional regulator Runx2 has essential roles in chondrocytes and osteoblasts, central to the coordinated development of cartilage and bone. However, the regulatory mechanisms underlying Runx2's roles in skeletal programming are not well understood. Here, we performed an integrative analysis of Runx2-DNA binding and chromatin accessibility in vivo and identified cell type-distinct chromatin accessibility underlying Runx2 roles in osteoblasts and chondrocytes. Examination of transcriptome in skeletal cells转录调节因子Runx2（Runx2）在软骨细胞与成骨细胞中发挥关键作用，是软骨与骨骼协同发育的核心调控因素。然而，Runx2在骨骼发育编程中行使功能的调控机制目前仍未得到充分阐释。本研究针对体内Runx2与DNA的结合情况及染色质可及性开展整合分析，鉴定出成骨细胞与软骨细胞中与Runx2功能相关的细胞类型特异性染色质可及性特征。对骨骼细胞转录组的分析。