A Lipidomic Exploration of the Effects of High-Intensity Interval Exercise in Healthy Men after Metformin Intake

We have previously found that high-intensity interval
exercise
(HIIE) affected metformin pharmacokinetics compared with rest. In
this scenario, changes in individual lipids could play an important
role. The prolonged responses of the lipidome to HIIE have not been
explored. This study aimed to explore differences in the plasma lipidomic
profiles between HIIE and rest, both under metformin treatment. Nine
healthy males participated in two sessions where they received 1,000
mg of metformin. In session A, they performed HIIE at an average intensity
of 67% of the maximum heart rate for a total duration of 76 min, whereas
in session B, they rested. Plasma was collected before taking metformin
and during each session (in total, 14 time points spanning 12 h).
Samples were analyzed through lipidomics using mass spectrometry.
We found several variations in the lipid profiles due to HIIE, which
persisted until 4 h post-exercise. Main discriminant lipid classes
were fatty acids, acyl carnitines, glycerophosphocholines, sphingomyelins,
and triacylglycerols. These changes were followed in time up to 12
h, showing the effect of the meals taken during the session. We hypothesize
the changes are a synergic effect of HIIE and metformin in the lipidome,
with the effect of HIIE being the predominant.

既往研究显示，与静息状态相比，高强度间歇运动（high-intensity interval exercise, HIIE）会影响二甲双胍（metformin）的药代动力学。在此背景下，个体脂质的变化可能发挥重要作用，但目前尚未有研究探讨脂质组对高强度间歇运动的长期响应特征。
本研究旨在探究二甲双胍干预下，高强度间歇运动与静息状态的血浆脂质组谱差异。本研究共招募9名健康男性受试者，开展两次实验流程，两次实验均为受试者服用1000mg二甲双胍。在实验A中，受试者以平均67%最大心率的强度进行总时长76分钟的高强度间歇运动；实验B中受试者保持静息。分别在服用二甲双胍前及两次实验过程中采集血浆样本，总计14个时间点，覆盖12小时时长。
采用脂质组学结合质谱法对样本进行分析。
研究结果发现，高强度间歇运动可导致血浆脂质谱发生多项显著变化，且该变化可持续至运动后4小时。主要的判别脂质类别包括脂肪酸、酰基肉碱、甘油磷酸胆碱、鞘磷脂及三酰甘油。本研究对上述变化进行了长达12小时的动态追踪，观察到实验期间进食的餐食对脂质变化的影响。我们提出假说：上述脂质组的变化是高强度间歇运动与二甲双胍的协同效应，其中高强度间歇运动的作用占主导地位。