CISI C57BL/6 mouse coronal sections containing MOp - October 2020 - tissue section 5

This dataset is part of an experiment demonstrating Composite In Situ Imaging (CISI) on whole coronal sections from a bisected mouse brain. The experiment profiled 37 genes through a combination of composite measurements and validation measurements in 12 tissue sections, with the validation measurements varying in each section. Both types of measurements were made using cyclic HCR-FISH, with composite measurements consisting of multiple probes simultaneously staining with the same dye in a given round of imaging. Composite measurements were decompressed to recover predicted images for individual genes, and these were compared with validation images and used for downstream analysis (e.g. cell type clustering).

该数据集是一项在对半切开的小鼠大脑全冠状切片上展示复合原位成像（Composite In Situ Imaging, CISI）的实验的一部分。实验通过复合测量与验证测量相结合的方式，在12个组织切片中分析了37个基因，且每个切片中的验证测量有所不同。两种测量均采用循环HCR-FISH技术完成，其中复合测量在特定成像轮次中由多个探针使用同一染料同时染色构成。复合测量经解压缩后可恢复单个基因的预测图像，这些图像与验证图像进行了比较，并用于下游分析（如细胞类型聚类）。