Seed-set under natural conditions

To estimate the number of ovules and the seeds per fruit under natural conditions at each site (ridge, slope, and valley), we examined the plants growing within and nearby the quadrats over 2 years (2010 and 2011). The total number of pin and thrum plants examined was 78 and 79 during 2010 and 170 and 157 during 2011, respectively. We collected several fruits from each plant (2.8 on average) during 2010, and one fruit per plant during 2011. The total number of fruits collected from the pin and thrum plants was 233 and 204 during 2010 and 170 and 157 during 2011, respectively. The fruits were collected just before dehiscence, and the number of seeds and undeveloped seeds or ovules were counted using a microscope.

为估算各生境（垄地、坡地与河谷）自然条件下单果的胚珠与种子数量，我们于2010至2011年两年间，对样方内外的植株开展了调查。2010年调查的针状花柱型（pin）与短柱型（thrum）植株数量分别为78株与79株，2011年该数值则分别为170株与157株。2010年我们从每株植株上采集多枚果实（平均单株采集2.8枚），2011年则改为每株仅采集1枚果实。2010年从针状花柱型与短柱型植株上采集的果实总数分别为233枚与204枚，2011年该数值则分别为170枚与157枚。所有果实均于开裂前即刻采集，并借助显微镜统计种子、未发育种子及胚珠的数量。