Regulartory effect of HNRNPL and LARP on RNA expression in LNCaP prostate cancer cells

Analysis of RNA expression in LNCaP prostate cancer cells treated with different siRNAs to define the regulatory effect of HNRNPL and LARP on RNA expression. LNCaP prostate cancer cells were treated with the control siRNA oligos and the siRNA oligos that knockdown the expression of HNRNPL and LARP. The polyA-RNA expression difference upon different siRNA oligo treatment was evaluted.

本研究旨在明确异质核核糖核蛋白L（HNRNPL）与La相关蛋白（LARP）对RNA表达的调控作用，为此使用不同小干扰RNA（siRNA）处理LNCaP前列腺癌细胞以分析其RNA表达情况。实验中，分别以对照siRNA寡核苷酸链，以及可敲低HNRNPL与LARP表达的siRNA寡核苷酸链处理LNCaP前列腺癌细胞，并对不同siRNA寡核苷酸链处理后细胞内多聚腺苷酸化RNA（polyA-RNA）的表达差异进行了评估。