Biodegradation of Cyclohexylamine by Brevibacterium oxydans IH-35A

A bacterial strain capable of growing on cyclohexylamine (CHAM) was isolated by using enrichment and isolation techniques. The strain isolated, strain IH-35A, was classified as a member of the genus Brevibacterium. The results of growth and enzyme studies are consistent with degradation of CHAM via cyclohexanone (CHnone), 6-hexanolactone, 6-hydroxyhexanoate, and adipate. Cell extracts obtained from this strain grown on CHAM contained CHAM oxidase, and the model for CHAM oxidation by this enzyme was similar to the model for deamino oxidation of amine by amine oxidase.

本研究通过富集分离技术，筛选得到一株可依托环己胺（cyclohexylamine，CHAM）生长的细菌菌株。该分离菌株IH-35A被归类为短杆菌属（Brevibacterium）的成员。生长实验与酶学研究结果表明，该菌株对CHAM的降解途径依次为环己酮（cyclohexanone，CHnone）、6-己内酯、6-羟基己酸酯与己二酸。以CHAM培养该菌株所获得的细胞提取物中含有环己胺氧化酶（CHAM oxidase），该酶催化CHAM氧化的反应模型与胺氧化酶介导的胺类脱氨基氧化反应模型相似。