Microbial metagenome analysis of soybean root nodules. rhizosphere metagenome

Recently, high-throughput sequencing technology is rapidly improving in quality, speed and cost. It is, therefore, becoming more widely used to study on whole bacterial communities. Rhizobial inoculation was often unsuccessful in legume fields, because soil native strains highly competed with the introduced inoculants. Conventional methods to monitor the behavior of inoculants are likely impractical and time-consuming in agricultural fields. Thus, We analyzed metagenome of the bacterial community associated with root nodules of soybean (Glycine max [L.] Merr., var. Tachinagaha) in order to descriminate the inoculant population from soil native population of soybean bradyrhizobia in an agricultural soil by mapping metagenome reads to the representative soybean bradyrhizobial genomes.

近年来，高通量测序技术（high-throughput sequencing technology）在测序质量、速度与成本控制层面均实现了快速提升。正因如此，该技术正被愈发广泛地应用于完整细菌群落的研究当中。在豆科作物田的根瘤菌接种工作中，接种效果往往不佳，其原因在于土壤中的土著根瘤菌菌株会与引入的接种菌株发生激烈竞争。传统用于监测接种菌株行为的方法，在农田场景下往往既不具备实用性又耗时耗力。有鉴于此，本研究针对大豆（Glycine max [L.] Merr.，品种Tachinagaha）根瘤相关细菌群落的宏基因组（metagenome）展开分析，通过将宏基因组测序读段（reads）比对至代表性大豆慢生根瘤菌基因组，以区分农田土壤中接种的大豆慢生根瘤菌种群与土著大豆慢生根瘤菌种群。