TGFBI is upregulated in ectopic endometrial stromal cells and under hypoxic conditions in endometriosis.. TGFBI is upregulated in ectopic endometrial stromal cells and under hypoxic conditions in endometriosis.

The objective of the study was to compare the transcriptome of the primary eutopic and ectopic endometrial stromal cells isolated from paired eutopic endometrium and peritoneal lesions from women with endometriosis (N = 5) exposed to normoxic (20% O2) or hypoxic (1% O2) conditions for 48 hours. Induced expression of TGFBI was found in ectopic cells and ectopic & eutopic cells exposed to hypoxia at both RNA and protein (secreted to the culture media) levels. TGFBI gene and protein expression ex vivo in euoptic endometrium of women with endometriosis and controls (women without endometriosis) was found to be increased in the proliferative phase of menstrual cycle compared to secretory phase. Overall design: Compare the expression of mRNAs in lysates of differently treated eutopic and ectopic endometrial stromal cells

本研究旨在对比子宫内膜异位症患者（N=5）配对在位子宫内膜与腹膜异位病灶分离得到的原代在位、异位子宫内膜间质细胞，在常氧（20% O₂）或低氧（1% O₂）条件下培养48小时后的转录组。研究发现，经低氧暴露的异位细胞以及异位与在位细胞中，转化生长因子β诱导蛋白（TGFBI）在RNA及蛋白质（分泌至培养基）水平均出现诱导表达。此外，离体状态下，子宫内膜异位症患者与对照组（无子宫内膜异位症女性）的在位子宫内膜中，TGFBI基因与蛋白质表达在月经增殖期较分泌期升高。整体实验设计：对比不同处理条件下的在位与异位子宫内膜间质细胞裂解物中的mRNA表达水平。