Data for: Soy Hydrolysate Mimic Autocrine Growth Factors Effect of Conditioned Media to Promote Single CHO-K1 Cell Proliferation

The increasingly competitive biopharmaceutical industry requires companies to focus on rapid and low-cost cell line development. Single-cell cloning (SCC) is a critical and high value process for cell line development, and typically problematic because single cell proliferates slowly when cultivated at low cell densities. Conditioned media (CM) provide autocrine growth factors to facilitate single cell proliferation, thus improve SCC efficiency. However, conditioned media (CM) are not a feasible solution for industrial cell line development due to variation and cross contamination concerns. Here, we have found an improvement in the SCC efficiency similar to CM when soy hydrolysate was supplemented in SCC media. Therefore, we concluded that hydrolysate can mimic the autocrine growth factor(s) effect to improve cloning efficiency observed in CM.

日益激烈的生物制药行业竞争要求企业聚焦于快速且低成本的细胞系开发。单细胞克隆（Single-cell cloning, SCC）是细胞系开发中至关重要且高价值的工艺环节，但该环节往往存在难题：当以低细胞密度培养时，单个细胞增殖速度缓慢。条件培养基（Conditioned media, CM）可提供自分泌生长因子以促进单个细胞增殖，进而提升SCC效率。然而，由于存在批次差异与交叉污染隐患，条件培养基（CM）并不适用于工业化细胞系开发流程。本研究发现，在SCC培养基中添加大豆水解物时，可获得与CM相似的SCC效率提升效果。据此我们得出结论：水解物可模拟CM中的自分泌生长因子效应，从而提升克隆效率。