Expression data from outflow region at E9.5. Mus musculus

A high concentration of maternal retinoic acid (RA), the active derivative of vitamin A, is well known as a teratogenic agent which can cause developmental defects. Our previous studies have shown that the maternal administration of RA to mice within a narrow developmental window induces outflow tract (OFT) septum defects, which closely resembles human transposition of the great arteries (TGA), although the responsible factors and pathogenic mechanisms of the TGA induced by RA still remain unknown. To identify the candidate genes involved in the altered development of the OFT in RA-induce TGA model, we isolated the RNA from E9.5 control and RA-treated OFT regions, and performed a microarray analysis. Overall design: Approximately 200 outflow tract region were isolated from control and RA-treated embryos (embryonic day 9.5). We sought to obtain the candidate genes involved in the altered development of the OFT in RA-induce heart defects.

高浓度的母体维甲酸（retinoic acid, RA）作为维生素A的活性衍生物，是一类公认的致畸剂，可引发胚胎发育缺陷。我们此前的研究表明，在小鼠发育的狭窄窗口期向孕鼠施加RA，可诱导流出道（outflow tract, OFT）间隔缺损，其表型与人类大动脉转位（transposition of the great arteries, TGA）高度相似，但RA诱导TGA的致病因子及分子机制仍未明确。为筛选RA诱导TGA模型中OFT发育异常相关的候选基因，我们从胚胎第9.5天（E9.5）的对照组与RA处理组的OFT区域中提取RNA，并开展了基因芯片分析（microarray analysis）。实验设计：从对照组与RA处理组的胚胎（胚胎第9.5天）中分离约200份流出道组织，本研究旨在筛选RA诱导心脏发育缺陷中OFT发育异常相关的候选基因。