The role of TEFM in mitochondrial gene expression [smallRNA]. Mus musculus

We investigated the role of TEFM in vivo by creating heart- and skeletal-muscle specific knockout of this gene. We identified that TEFM is required for the processivity of POLRMT and its loss leads to significantly decreased mitochondrial transcription. Overall design: Small RNA from isolated heart mitochondria from four control mice (L/L) and four Tefm knockout mice (L/L, cre), was sequenced and analysed for differential expression.

本研究通过构建该基因的心脏与骨骼肌特异性敲除模型，在体内探究了TEFM的生物学作用。研究证实，TEFM是POLRMT持续合成活性所必需的，该基因的缺失会显著降低线粒体转录水平。实验设计概述：从4只对照小鼠（L/L基因型）和4只Tefm敲除小鼠（L/L，cre基因型）的分离心脏线粒体中提取小RNA，进行测序并分析差异表达情况。