Transcriptome sequence of hTERT-HPNE and PANC-1 cells upon hydrogen peroxide treatment

To explore the whole transcriptional regulation of lncRNA and mRNA expression in hydrogen peroxide (H2O2) signaling, we used human pancreatic normal epithelial cell line hTERT-HPNE and pancreatic cancer cell line PANC-1 for H2O2 treatment and utilized transcriptome sequence to analyze the change of lncRNA and mRNA transcripts. Then we predicted the potential transcription factor for these significantly changed transcripts. hTERT-HPNE cells with or without 0.3 mM H2O2 treatment for 12h, PANC-1 cells with or without 1.0 mM H2O2 treatment for 12h

为探究过氧化氢（hydrogen peroxide, H₂O₂）信号通路中长链非编码RNA（long non-coding RNA, lncRNA）与信使RNA（messenger RNA, mRNA）的完整转录调控机制，我们选用人胰腺正常上皮细胞系hTERT-HPNE及胰腺癌细胞系PANC-1进行过氧化氢处理，并通过转录组测序分析两类转录本的表达变化。随后，我们针对上述表达显著变化的转录本，预测其潜在的转录因子。实验分组如下：hTERT-HPNE细胞经0.3 mM过氧化氢处理12小时组与未处理对照组；PANC-1细胞经1.0 mM过氧化氢处理12小时组与未处理对照组。