Gene expression differences between activated B cells expressing GL7 and not in Peyer´s patches

We report two populations of activated germinal centre B cells in the Peyer´s patches that are distinguished by their expression of GL7 or not. Using an antigen-specific system, we sort GL7+ and GL7- activated cells and performe RNASeq. We also included GL7 expressing B cells activated against the gut microbiota, and resting B cells from Peyer´s patches and spleen. GFP expressing NP-specific B cells from spleen were transfered into recipient wild type C57Bl/6 mice and the mice were orally immunized with NP conjugated to cholera toxin (NP-CT). Antigen-specific cells were isolated based on GFP and GL7 expression and compared to endogenous GL7+ and GL7- cells.

本研究报道了派尔集合淋巴结（Peyer´s patches）中两类以是否表达GL7（GL7）为区分标志的活化生发中心B细胞群。本研究借助抗原特异性实验体系，对GL7阳性与GL7阴性的活化细胞进行分选并开展RNA测序（RNASeq）。此外，本研究还纳入了针对肠道菌群活化的表达GL7的B细胞，以及源自派尔集合淋巴结与脾脏的静息B细胞。将脾脏来源的表达绿色荧光蛋白（GFP）的NP特异性B细胞移植至野生型C57Bl/6受体小鼠体内，随后以霍乱毒素偶联NP（NP-CT）对小鼠实施口服免疫。本研究基于GFP与GL7的表达情况分离抗原特异性细胞，并将其与内源性GL7阳性及GL7阴性细胞进行对比分析。