Transcriptional profiles of genes in the early stage of osteoclastogenesis. Mus musculus

Osteoclast (OC) differentiation undergoes a two-step process: commitment of hematopoietic progenitor cells to tartrate-resistant acid phosphatase (TRAcP) positive OC precursors (OCPs), and fusion of OCPs into multinucleated OCs. In order to identify transcriptional profiles of genes in the transitional phase between OC commitment and fusion in OCG, Affymetrix® Mouse Gene 1.0 ST arrays were performed on total RNA extracted from mouse (SV129/BL6 ) monocytes and pre-osteoclasts (pre-OCs), primed with macrophage colony-stimulated factor (M-CSF) or M-CSF and soluble recombinant receptor activator of NF-кB ligand (sRANKL), respectively. The analysis identified 656 RANKL-up or down-regulated in the early stage of osteoclastogenesis. Overall design: Monocytes isolated from mouse bone marrow were stimulated with M-CSF and soluble RANKL (m + r), or M-CSF alone (m).

破骨细胞（Osteoclast, OC）的分化分为两个阶段：造血祖细胞先定向分化为抗酒石酸酸性磷酸酶（tartrate-resistant acid phosphatase, TRAcP）阳性的破骨细胞前体（OCPs），随后破骨细胞前体融合形成多核破骨细胞。为鉴定破骨细胞生成过程中OC定向与融合过渡期的基因转录谱，研究人员对从小鼠（SV129/BL6）单核细胞及前破骨细胞（pre-OCs）中提取的总RNA开展了Affymetrix®小鼠基因1.0 ST芯片检测：其中单核细胞分别经巨噬细胞集落刺激因子（macrophage colony-stimulated factor, M-CSF）单独预处理，或同时经M-CSF与可溶性重组核因子κB受体活化因子配体（soluble recombinant receptor activator of NF-кB ligand, sRANKL）处理。该分析共鉴定出656个在破骨细胞生成早期受RANKL调控（上调或下调）的基因。整体实验设计：从小鼠骨髓中分离的单核细胞，分别采用M-CSF联合可溶性RANKL（m + r）、或单独使用M-CSF（m）进行刺激。