Size does matter”– Improvement of High throughput DNA metabarcoding by size & order sorting of malaise trap samples

Using reliably identified DNA barcode sequence data of the German barcode projects and a CUSTOM BLAST approach, we were able to detect a total of 566 Barcode Index Numbers (BINs) within all sorted malaise trap fractions. Analysis of the unsorted fraction revealed 48% less recovered BINs. Furthermore, we demonstrate that a combination of inexpensive “low depth” sequenced size pre-sorted fractions will result in higher BIN recovery than application of expensive “high depth” sequencing with (960,000 reads) for analysis of an unsorted sample.

本研究借助经可靠鉴定的德国条形码项目（German barcode projects）DNA条形码（DNA barcode）序列数据与自定义BLAST分析方法，在所有经分选的马氏诱捕器（malaise trap）样本组分中共检测到566个条形码索引号（Barcode Index Numbers, BINs）。对未分选组分的分析显示，其可恢复的BINs数量较分选组低48%。此外，本研究证实：相较于采用单次测序读段量达960,000条的昂贵高深度测序方案分析未分选样本，将低成本的“低深度”测序尺寸预分选组分进行组合，可获得更高的BINs回收率。