Beta vulgaris subsp. vulgaris cultivar:KDH13 Genome sequencing and assembly

The genome of the sugar beet (Beta vulgaris subsp. vulgaris) doubled haploid line (KDH13) was sequenced using Illumina next generation sequencing HiSeq2000 platform. This line (PI663862) was released by USDA-ARS as genetic stock resistant to beet curly top. The whole genome sequencing shotgun (WGS) of two genomic libraries; a short-insert paired end (PE) and a 2kb-insert mate-pair (MP) resulted in 82.9 Gb of sequence data. BvvSeq-1 sugar beet genome assembly released in May 2013 is the first public assembly of a public sugar beet germplasm (KDH13). There are other sugar beet whole genome sequencing efforts elsewhere including the draft RefBeet_0.9 sequence released by the European BeetSeq consortium. Additionally, there are many published genetic linkage maps, ESTs and BACs sequencing projects that have been implemented with an aim of developing advanced genetics knowledge for a better crop.

研究人员采用Illumina下一代测序HiSeq2000平台，对甜菜（Beta vulgaris subsp. vulgaris）双单倍体系（doubled haploid line，KDH13）的基因组完成了测序。该品系（PI663862）由USDA-ARS发布，为抗甜菜曲顶病的遗传种质资源。针对两种基因组文库开展的全基因组鸟枪法测序（whole genome sequencing shotgun，WGS）——即短插入片段双端测序（short-insert paired end，PE）文库与2kb插入片段配对末端测序（mate-pair，MP）文库——共产生82.9 Gb的序列数据。2013年5月发布的BvvSeq-1甜菜基因组组装版本，是首个针对公共甜菜种质（KDH13）的公开基因组组装结果。全球范围内还存在其他甜菜全基因组测序相关研究，包括欧洲甜菜测序联盟（European BeetSeq consortium）发布的RefBeet_0.9草图序列。此外，已有诸多已发表的遗传连锁图谱、表达序列标签（Expressed Sequence Tags，ESTs）及细菌人工染色体（Bacterial Artificial Chromosomes，BACs）测序项目，旨在深化甜菜遗传学研究，培育更优良的作物品种。