ASA³P Software & Database volume

ASA³P is an automatic and highly scalable assembly, annotation and higher-level analyses pipeline for closely related bacterial isolates. https://github.com/oschwengers/asap

ASA³P is a fully automatic, locally executable and scalable assembly, annotation and higher-level analysis pipeline creating results in standard bioinformatics file formats as well as sophisticated HTML5 documents. Its main purpose is the automatic processing of NGS WGS data of multiple closely related isolates, thus transforming raw reads into assembled and annotated genomes and finally gathering as much information on every single bacterial genome as possible. Per-isolate analyses are complemented by comparative insights. Therefore, the pipeline incorporates many best-in-class open source bioinformatics tools and thus minimizes the burden of ever-repeating tasks. Envisaged as a preprocessing tool it provides comprehensive insights as well as a general overview and comparison of analysed genomes along with all necessary result files for subsequent deeper analyses. All results are presented via modern HTML5 documents comprising interactive visualizations.

Schwengers et al, 2020 PLOS Comp Bio DOI:10.1371/journal.pcbi.1007134

ASA³P 是一款面向近缘细菌分离株的自动化、高可扩展性基因组组装、注释及高阶分析流程。https://github.com/oschwengers/asap

ASA³P 是一款完全自动化、支持本地执行且具备良好可扩展性的基因组组装、注释与高阶分析流程，可输出标准生物信息学文件格式的结果，同时生成精美的HTML5文档。其核心功能为自动化处理多株近缘分离株的下一代全基因组测序（NGS WGS）数据，将原始测序读段转化为组装并注释完成的基因组，最终尽可能全面地获取每一株细菌基因组的相关信息。单株分离株的分析还辅以比较基因组学视角。为此，该流程集成了多款业界领先的开源生物信息学工具，从而大幅减轻重复分析任务带来的工作负担。作为一款预处理工具，它不仅可提供全面的分析结果，还能为待分析基因组提供通用概览与比较分析内容，同时附带所有用于后续深度分析的必要结果文件。所有分析结果均通过搭载交互式可视化功能的现代化HTML5文档进行展示。

Schwengers 等人，2020年《公共科学图书馆·计算生物学》（PLOS Comp Bio），DOI:10.1371/journal.pcbi.1007134