Table_2_Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylated Proteins in Fusarium oxysporum.XLSX

Protein lysine 2-hydroxyisobutyrylation (Khib), a new type of post-translational modification, occurs in histones and non-histone proteins and plays an important role in almost all aspects of both eukaryotic and prokaryotic living cells. Fusarium oxysporum, a soil-borne fungal pathogen, can cause disease in more than 150 plants. However, little is currently known about the functions of Khib in this plant pathogenic fungus. Here, we report a systematic analysis of 2-hydroxyisobutyrylated proteins in F. oxysporum. In this study, 3782 Khib sites in 1299 proteins were identified in F. oxysporum. The bioinformatics analysis showed that 2-hydroxyisobutyrylated proteins are involved in different biological processes and functions and are located in diverse subcellular localizations. The enrichment analysis revealed that Khib participates in a variety of pathways, including the ribosome, oxidative phosphorylation, and proteasome pathways. The protein interaction network analysis showed that 2-hydroxyisobutyrylated protein complexes are involved in diverse interactions. Notably, several 2-hydroxyisobutyrylated proteins, including three kinds of protein kinases, were involved in the virulence or conidiation of F. oxysporum, suggesting that Khib plays regulatory roles in pathogenesis. Moreover, our study shows that there are different Khib levels of F. oxysporum in conidial and mycelial stages. These findings provide evidence of Khib in F. oxysporum, an important filamentous plant pathogenic fungus, and serve as a resource for further exploration of the potential functions of Khib in Fusarium species and other filamentous pathogenic fungi.

蛋白质赖氨酸2-羟基异丁酰化（lysine 2-hydroxyisobutyrylation, Khib）作为一种新型翻译后修饰（post-translational modification, PTM），既存在于组蛋白与非组蛋白中，在真核与原核活细胞的几乎所有生命活动中均发挥重要作用。尖孢镰刀菌（Fusarium oxysporum）是一种土传真菌病原物，可侵染150余种植物引发病害，但目前学界对该植物病原真菌中Khib的功能尚所知甚少。本研究对尖孢镰刀菌中的2-羟基异丁酰化蛋白质组开展了系统性分析，共在该菌的1299个蛋白质中鉴定到3782个Khib修饰位点。生物信息学分析显示，2-羟基异丁酰化蛋白质参与多种生物学过程与功能，且分布于不同的亚细胞区域；富集分析结果表明，Khib参与核糖体、氧化磷酸化及蛋白酶体等多条通路。蛋白质相互作用网络分析显示，2-羟基异丁酰化蛋白质复合物参与多种相互作用过程。值得注意的是，包括3种蛋白激酶（protein kinases）在内的多种2-羟基异丁酰化蛋白质参与了尖孢镰刀菌的毒力调控与产孢过程，提示Khib在病原菌致病过程中发挥调控作用。此外，本研究发现尖孢镰刀菌在分生孢子与菌丝阶段的Khib修饰水平存在显著差异。本研究结果为重要丝状植物病原真菌尖孢镰刀菌中的Khib修饰提供了系统性研究依据，同时可为后续探究镰刀菌属及其他丝状病原真菌中Khib的潜在功能提供宝贵的研究资源。