Amplicon sequencing of different regions of metastatic melanomas. Amplicon sequencing of different regions of metastatic melanomas

Genetic heterogeneity can provide tumors with opportunities for therapy evasion, however the degree of genetic heterogeneity within metastatic melanomas has not been thoroughly investigated. We therefore isolated DNA from different regions of formalin fixed paraffin embedded metastatic melanoma tissue samples and subjected them to amplicon sequencing-based profiling of mutations in a panel of well known cancer genes using the Ion Ampliseq Cancer Panel.

遗传异质性可为肿瘤带来治疗逃逸的契机，但目前尚未对转移性黑色素瘤内部的遗传异质性程度开展全面深入的研究。为此，我们从福尔马林固定石蜡包埋（formalin fixed paraffin embedded）转移性黑色素瘤组织样本的不同区域中提取DNA，并采用Ion Ampliseq癌症基因检测面板（Ion Ampliseq Cancer Panel），基于扩增子测序技术对一组公认癌症基因的突变特征进行谱分析。