Effect of CuCl2 on transcriptional expression of MDA-MB-468 cells. Effect of CuCl2 on transcriptional expression of MDA-MB-468 cells

To investigate the effects of disrupting Cu homeostasis on MDA-MB-468 cells in vitro, we treated cells with DCAC50, tetrathiomolybdate, or DMSO (control) or sDMEM for 24 hours Overall design: Following RNA extraction, ribo-minus library generation, and sequencing, we then performed differential expression analysis of extracted RNA, comparing DCAC50- or tetrathiomolybdate-treated cells to DMSO-treated

为探究铜稳态紊乱对体外培养的MDA-MB-468细胞的影响，我们将细胞分别以DCAC50、四硫钼酸盐(tetrathiomolybdate)、二甲基亚砜(DMSO，对照)及无血清DMEM(sDMEM)处理24小时。实验设计概述：完成RNA提取、核糖体去除型文库构建及测序后，我们对提取的RNA开展差异表达分析，将经DCAC50或四硫钼酸盐处理的细胞与经DMSO处理的细胞进行对比。