The effect of whole-glucan particles on the gene expression of polarized M2 bone-derived macrophages. Mus musculus

Bone-marrow macrophages polarized to M2 phenotype are immunosuppressive. Interestingly, treatment with whole-glucan particles converts M2 macrophages to M1 phenotype with an anti-tumor phenotype. In this study, the effect of WGP treatment for 6 hours on the gene expression of M2 macrophages was assessed. Overall design: Polarized M2 bone-marrow derived macrophages were incubated for 6 hours in media with or without WGP, followed by RNA extraction.

极化为M2表型(M2 phenotype)的骨髓巨噬细胞具有免疫抑制功能。值得关注的是，全葡聚糖颗粒(whole-glucan particles, WGP)处理可将M2巨噬细胞转化为具有抗肿瘤表型的M1表型。本研究评估了WGP处理6小时对M2巨噬细胞基因表达的影响。实验设计概况：将极化为M2表型的骨髓来源巨噬细胞置于含有或不含有WGP的培养基中孵育6小时，随后进行RNA提取。