Putative mitosomal proteins classified by predicted function: protein modification, cztosceletal and motor proteins.

Mascot score, Mascot total ion score for the identified protein. Coverage, number of unique peptides per identified protein. MiD, mitosomal distribution. Proteins are marked “Y” if their distributions in fractions #3 and #4 of the Optiprep gradient (measured by the iTRAQ ratio) were within the range between Cpn10 and IscU and the window that extended in both directions by half of the distance between these markers. Proteins with ratios outside of this range are indicated with “N”. TargetP and PsortII were used to predict the subcellular location of Giardia proteins. S, secretory; N, non-secretory; M, mitochondrial; O, other. Exp. ver., experimental verification of protein localization using the pONDRA expression vector. The recombinant tagged proteins were localized by fluorescence microscopy. M, mitosome; ER, endoplasmic reticulum; O, other; ? inconclusive. MEMSAT3 and TMHMM were used to predict transmembrane domains. SGP, predicted soluble proteins are marked with number sign (#). Asterisk (*) is used where no data were available. (**) transformed Giardia did not express the recombinant tagged protein.

Mascot评分（Mascot score）：针对鉴定蛋白的Mascot总离子评分（Mascot total ion score）。
Coverage：单个鉴定蛋白的唯一肽段数量。
MiD：纺锤剩体分布（mitosomal distribution）。
若某蛋白在Optiprep密度梯度离心（Optiprep gradient）的第3、4组分中的分布（通过同量异位素标记相对和绝对定量（iTRAQ）比值测定）处于伴侣蛋白10（Cpn10）与铁硫簇组装蛋白U（IscU）之间的区间，且向两侧延伸至这两个标记物间距一半的范围内，则标记为“Y”；分布超出该范围的蛋白则标记为“N”。
采用靶肽预测工具（TargetP）与亚细胞定位预测工具（PsortII）对贾第虫（Giardia）蛋白的亚细胞定位进行预测，分类标准如下：S代表分泌型蛋白，N代表非分泌型蛋白，M代表线粒体定位型蛋白，O代表其他类型蛋白。
Exp. ver.：指采用pONDRA表达载体（pONDRA expression vector）对蛋白定位进行的实验验证。通过荧光显微镜对带有标签的重组蛋白进行定位观测，结果分类如下：M代表纺锤剩体（mitosome），ER代表内质网（endoplasmic reticulum），O代表其他类型，?代表结果不确定。
采用MEMSAT3（MEMSAT3）与TMHMM（TMHMM）工具预测蛋白的跨膜结构域。
SGP：预测的可溶性蛋白以井号（#）标注。
星号（*）用于标注无可用数据的条目。
(**)：表示转化后的贾第虫未表达带有标签的重组蛋白。