Characterization of a TUTase/nuclease complex required for Drosophila gametogenesis

Total RNAs were cloned from wt, Dis3L2 and Tailor mutant testis tissues to study the role of Tailor and Dis3L2 TUTase/nuclease complex Overall design: Replicated total RNA samples from Dis3L2 and Tailor single mutant, and Dis3L2/Tailor double mutant and WT (w1118) testes

为探究Tailor与Dis3L2 尿苷酰转移酶/核酸酶（TUTase/nuclease）复合物的功能，研究人员从野生型（wild type, wt）、Dis3L2突变体及Tailor突变体的睾丸组织中克隆总RNA。实验设计概述：设置生物学重复，分别采集Dis3L2单突变体、Tailor单突变体、Dis3L2/Tailor双突变体以及野生型（w1118）的睾丸总RNA样本。