Genomic diversity of a pneumococcal clonal complex, 199

Comparative genomic hybridisation of Streptococcus pneumoniae isolates from a single clonal complex, in order to determine genomic diversity. Isolates were selected from a range of tissue types and serotypes in order to cover the full diversity of the clone, and also in order to try and identify tissue-specific genes Biological replicates: 19 clonal complex 199 S. pneumoniae isolates. One clonal complex 180 isolate used as an outgroup. Independently grown and isolated. One isolate per array

本研究针对单一克隆复合体（clonal complex）的肺炎链球菌（Streptococcus pneumoniae）分离株开展比较基因组杂交（comparative genomic hybridisation, CGH）实验，旨在解析该克隆的基因组多样性。研究人员从多种组织类型与血清型中筛选分离株，以覆盖该克隆的全部遗传多样性，并试图鉴定组织特异性基因。本次实验的生物学重复样本为19株克隆复合体199型肺炎链球菌分离株，另选取1株克隆复合体180型分离株作为外类群（outgroup）；所有分离株均经独立培养与分离，且每片基因芯片（array）仅使用一株分离株进行杂交。