RNA-seq of ileal resection margins of ulcerative colitis patients that had total colectomy and ileal pouch anal anastomosis

We previously showed that abnormal morphology phenotype of ileal Paneth cells (Paneth cell phenotype [PCP]; as a surrogate for PC function) correlate with genetics, microbiota compositions, and aggressive outcome in Crohn's disease (CD) patients. Given the shared genetics and clinical features between CD and ulcerative colitis (UC), we hypothesized that abnormal PCP also negatively modulates UC outcomes. As PCs has the highest density in the ileum, we further hypothesized that abnormal PCP from the terminal ileum could increase the risk of development of pouch complications after UC total colectomy and ileal pouch anal anastomosis (IPAA).

我们此前已证实，回肠潘氏细胞（Paneth cell, PC）的形态学异常表型——潘氏细胞表型（Paneth cell phenotype, PCP，可作为潘氏细胞功能的替代指标）——与克罗恩病（Crohn's disease, CD）患者的遗传学特征、菌群组成及不良临床结局显著相关。鉴于克罗恩病与溃疡性结肠炎（ulcerative colitis, UC）存在共有的遗传学特征与临床表型，我们提出假说：异常的潘氏细胞表型同样会对溃疡性结肠炎的临床结局产生负面影响。由于潘氏细胞在回肠中密度最高，我们进一步提出假说：经溃疡性结肠炎全结肠切除术联合回肠贮袋肛管吻合术（ileal pouch anal anastomosis, IPAA）治疗后，回肠末端的异常潘氏细胞表型可能会增加贮袋并发症的发生风险。