Additional file 1 of New approach for genomic characterisation of equine sarcoid-derived BPV-1/-2 using nanopore-based sequencing

Additional file 1. Detailed information on the clinical samples and the corresponding sequence variants. Clinical samples were processed in three different sample batches. While the primer set used for the first batch proved highly successful, the fraction of BPV-specific reads in some of the samples was low, indicating significant off-target amplification. The higher specificity of the V2 primer set significantly increased the fraction of BPV-specific reads.

附加文件1：临床样本及其对应序列变异体的详细信息。本次研究的临床样本被分为3个独立样本批次进行处理。尽管首批次使用的引物组扩增效果优异，但部分样本中牛乳头状瘤病毒（Bovine Papillomavirus，BPV）特异性读段的占比偏低，提示存在显著的非靶标扩增现象。V2引物组凭借更高的特异性，显著提升了BPV特异性读段的占比。