Data_Sheet_1_Ovine RAP1GAP and rBAT gene polymorphisms and their association with tail fat deposition in Hu sheep.PDF

Excessive fat deposition in the tail of sheep will affect its feed efficiency, which will increase the feeding cost. The purpose of this study was to identify the single nucleotide polymorphisms (SNPs) of RAP1GAP and rBAT genes by PCR amplification and Sanger sequencing, the SNPs were genotyped by KASP genotyping assays to evaluate their association with tail fat deposition traits. The results showed that two intronic mutations of g.13561 G > A and g.1460 T > C were found in RAP1GAP and rBAT, respectively. There were three genotypes of GG, AG, AA and CC, CT and TT at these two loci, respectively. Association analysis showed that g.13561 G > A of RAP1GAP was associated with tail width, tail fat weight and relative tail fat weight (P < 0.05). The g.1460 T > C of rBAT was associated with tail width and tail fat weight (P < 0.05). Different combinations of genotypes also differed significantly with tail fat deposition traits. In the tail fat tissue, the expression levels of RAP1GAP gene was significantly higher in small-tailed sheep than in big-tailed sheep, and the expression levels of rBAT gene was significantly higher in big-tailed sheep than in small-tailed sheep. In the liver, the expression levels of RAP1GAP and rBAT gene was significantly higher at 6 months than at 0 and 3 months. In conclusion, RAP1GAP and rBAT polymorphisms can be used as a candidate molecular marker to reduce tail fat deposition in sheep.

羊尾端脂肪沉积过量将影响其饲料利用率，进而增加饲养成本。本研究旨在通过聚合酶链反应扩增和Sanger测序技术识别RAP1GAP和rBAT基因的单核苷酸多态性（SNPs），并通过KASP基因分型检测评估其与尾脂沉积性状的相关性。研究发现，RAP1GAP和rBAT基因分别存在g.13561 G > A和g.1460 T > C的内含子突变。在这两个位点，分别存在GG、AG、AA和CC、CT、TT三种基因型。关联分析表明，RAP1GAP基因的g.13561 G > A突变与尾宽、尾脂重量及其相对尾脂重量（P < 0.05）相关。rBAT基因的g.1460 T > C突变与尾宽和尾脂重量（P < 0.05）相关。不同基因型的组合与尾脂沉积性状也存在显著差异。在尾脂组织中，与长尾羊相比，短尾羊中RAP1GAP基因的表达水平显著较高，而与短尾羊相比，长尾羊中rBAT基因的表达水平显著较高。在肝脏中，RAP1GAP和rBAT基因的表达水平在6个月时相较于0个月和3个月显著增加。综上所述，RAP1GAP和rBAT的多态性可作为候选分子标记，以降低羊的尾脂沉积。