Analysis of transgenerational effects on DNA copy number aberrations in male mice exposed to continuous 20mGy/day gamma-rays for 400 days (Primary screening for 0mGyL familly).. Mus musculus

Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. C57BL/6JNrs male mice were maintained in non-irradiated room for 400 days from 8 weeks of age. This study investigated the De novo mutation by comparing the parents and children. This is primary screening. This study was performed under contract with the Aomori Prefectural Government, Japan. Overall design: Two-condition experiment, C57 BL/6JNrs tail tissue non-irradiated male (0mGyLM), female (0mGyLF) and their progenies (0mGyL1 to 0mGyL6) vs. reference male mouse.

持续低剂量率（low dose-rate, LDR）γ射线辐照的跨代效应尚未得到充分研究。本研究选取8周龄起即在无辐照环境中饲养400天的C57BL/6JNrs雄性小鼠为实验材料，通过比对亲代与子代个体的基因序列，探究新生突变（de novo mutation）发生情况，本次实验属于初步筛选阶段。本研究依托与日本青森县政府签订的合作合约开展。实验设计：采用双条件对照实验方案，以未接受辐照的C57BL/6JNrs雄性小鼠（0mGyLM）、雌性小鼠（0mGyLF）及其子代（0mGyL1至0mGyL6）的尾部组织为实验组，与参考雄性小鼠进行对照分析。