Natural variation in the consequences of gene overexpression during osmotic stress [RNA-Seq]. Natural variation in the consequences of gene overexpression during osmotic stress [RNA-Seq]

We used RNA-Seq to measure transcript abundance in 4 Saccharomyces cerevisiae strains (BY4743, BC187, NCYC3290, and YPS128) from a diverse range of genetic lineages when growing in rich media (YPD) with 0.7M NaCl to characterize differential expression across strains in response to osmotic and ionic stress. Overall design: RNA-seq and transcriptome analysis of 4 strains (BY4743, BC187, NCYC3290, and YPS128) that were grown in YPD+ 0.7M NaCl. For each strain, 3 samples were collected: T0 (unstressed cells), T30 (30 mins after cells placed in 0.7M NaCl), and T3h (3 hours after cells placed in NaCl). These strains were collected in 3 biological replicates. The library for sample YPS128 Rep 3 T30 failed so this sample is missing and there are a total of 35 samples in the dataset.

本研究采用RNA测序（RNA-Seq）技术，对4株来自不同遗传谱系的酿酒酵母（Saccharomyces cerevisiae）菌株（BY4743、BC187、NCYC3290及YPS128）在添加0.7M氯化钠的丰富培养基（YPD）中培养时的转录本丰度进行检测，以表征不同菌株在渗透与离子胁迫响应中的差异表达模式。整体实验设计：对上述4株酿酒酵母开展RNA测序与转录组分析，所有菌株均在添加0.7M氯化钠的YPD培养基中培养。每株菌株设置3个采样时间点：T0（未接受胁迫的细胞）、T30（置于0.7M氯化钠中30分钟后的细胞）、T3h（置于氯化钠环境中3小时后的细胞），且每个时间点设置3次生物学重复。其中菌株YPS128的第3次重复T30样本的文库构建失败，导致该样本缺失，本数据集最终共包含35个样本。