SH1402375.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 为基于DNA的物种假说（Species Hypotheses，以下简称SH）的界定、识别、交流与研究提供了统一的操作框架。国际核酸序列数据库中的全部真菌内部转录间隔区（Internal Transcribed Spacer，简称ITS）序列，通过应用一套动态距离阈值（0.5%~3.0%）被聚类至近似物种级别的聚类单元。所有物种假说均会被赋予一个以数字对象标识符（Digital Object Identifier，简称DOI）为形式的唯一且稳定的标识名称，其分类学与生态学注释信息将通过分布式网络第三方注释工作完成核验。系统会结合该物种假说下所有序列的鉴定结果，尽可能将其与对应分类单元的名称及其所属分类层级（如门、纲、目等）进行关联匹配。研究人员会选定一条经自动或人工指定的序列作为该物种假说的代表序列。此类代表序列已对外发布（访问链接：https://unite.ut.ee/repository.php），可供科研群体用于本地序列相似性检索以及二代测序分析流程等科研场景。随着公共真菌ITS序列数据体量的持续增长，该系统与配套数据集将自动完成更新迭代。