Dataset for metagenomic analysis of bacterial community grown in sediments contaminated with pulp paper mill waste pollutants

Pulp and paper mills (PPMs) generate complex and recalcitrant chlorolignin pollutants in their waste stream, posing a significant environmental and health threat. To understand how microbial communities can help in the clean-up of chlorolignin waste discharged from PPMs, two sediment samples, i.e., PPS-1 and PPS-2, were collected from two sites (site-1 and site-2) to examine chemical contaminants and their effects on indigenous bacterial communities. Physicochemical and GC-MS analysis of sediment samples revealed the presence of heavy and various organic refractory and toxic pollutants. Simultaneously, high-throughput sequencing of the V3–V4 hypervariable region of the 16S rRNA genes of metagenome extracted from sediment samples, was conducted on the Illumina MiSeq platform. This dataset consists of raw sequencing data (fastq) analysis reports, pie charts, and barplots. In addition, it also consists of the analyzed rarefaction curve, heat map, and Krona chart. Taxonomic analysis of metagenomic sequence data revealed that Proteobacteria dominated both PPS-1 and PPS-2 at the phylum level. At the genus level, Thiobacillus was the most prevalent in sediment samples. These results highlight substantial differences in bacterial community diversity and taxonomic abundance between PPS-1 and PPS-2. This research offers valuable insights into the characteristics of contaminants, shifts in microbial communities, and their potential functions in chlorolignin waste-polluted sites.

制浆造纸厂（Pulp and paper mills, PPMs）的生产废水会产生复杂难降解的氯木质素污染物，对环境与人类健康构成严重威胁。为明确微生物群落如何助力制浆造纸厂排放的氯木质素废水的修复工作，研究人员从两个采样点（位点1与位点2）采集了两份沉积物样本（即PPS-1与PPS-2），用以检测其中的化学污染物及其对土著细菌群落的影响。
对沉积物样本的理化分析与气相色谱-质谱联用（GC-MS）检测结果显示，样本中存在多种重金属、难降解有机污染物及有毒物质。同时，研究人员依托Illumina MiSeq测序平台，对从沉积物样本中提取的宏基因组16S rRNA基因的V3-V4高变区进行了高通量测序。
本数据集包含原始测序数据（FASTQ格式）、分析报告、饼图与柱状图。此外，数据集还包含经分析得到的稀疏曲线、热图与Krona图表。
宏基因组测序数据的分类学分析结果显示，在门水平上，变形菌门（Proteobacteria）在PPS-1与PPS-2两份样本中均为优势菌群；在属水平上，硫杆菌属（Thiobacillus）为沉积物样本中的最优势菌属。上述结果表明，PPS-1与PPS-2两份样本的细菌群落多样性及分类学丰度存在显著差异。本研究为解析氯木质素污染位点的污染物特征、微生物群落演替规律及其潜在功能提供了重要参考。