RNA-sequencing analysis of differentiating wildtype and Ezh2 deficient immature mouse chondrocytes. Mus musculus

Objective: To evalute the role of Ezh2 in chondrocyte differentiation. Method: Ezh2 was inactivated in chondrocytes using the Col2a1-Cre driver in vivo. Immature mouse chondrocytes (IMC) were isolated from wildtype and Ezh2 deficient mice, and plated in micromass culture. Chondrocytes were differentiated and RNA was isolated at days 3, 7, and 14 of culture. Isolated RNA was subjected to RNA sequencing analysis. Results: Ezh2 deficient chondrocytes exhibit enhanced expression of osteogenic genes compared to wildtype cells. Overall design: Transcriptome analysis of wildtype and Ezh2-deficient chondrocytes at days 3, 7, and 14 of differentiation.

研究目的：本研究旨在评估Ezh2在软骨细胞分化中的作用。实验方法：体内借助Col2a1-Cre驱动系统（Col2a1-Cre driver）灭活软骨细胞内的Ezh2基因；从野生型及Ezh2缺陷型小鼠体内分离未成熟小鼠软骨细胞（Immature mouse chondrocytes, IMC）并进行微团培养；对分离得到的软骨细胞开展诱导分化，分别于培养第3、7、14天提取总RNA；将提取获得的总RNA进行RNA测序分析。实验结果：与野生型软骨细胞相比，Ezh2缺陷型软骨细胞的成骨基因表达水平显著上调。实验整体设计：对软骨细胞分化第3、7、14天的野生型与Ezh2缺陷型软骨细胞进行转录组分析。