CUT&Tag analysis of H3K9la in Cal27 and HN30 cells

To identify the genes directly regulated by H3K9la on a genome-wide scale, we performed CUT&Tag assays using a H3K9la specific antibody in CAL27 and HN30 with or without the lactic acid treatment, and performed CUT&Tag assays using a H3K9la specific antibody in HN30 under hypoxia condition. Refer to individual Series.

为在全基因组范围内鉴定受组蛋白H3赖氨酸9乳酸化（H3K9la）直接调控的基因，我们分别在经乳酸处理与未经乳酸处理的CAL27和HN30细胞系中，使用H3K9la特异性抗体开展CUT&Tag实验；同时在低氧条件下的HN30细胞系中，使用H3K9la特异性抗体开展CUT&Tag实验。具体信息请参阅各独立实验系列（Series）。