Cell surface nucleolin is a novel ADAMTS5 receptor mediating endothelial cell apoptosis. Cell surface nucleolin is a novel ADAMTS5 receptor mediating endothelial cell apoptosis

This study demonstrates that TS5-p45 induces endothelial cell (EC) apoptosis in a caspase-dependent manner via its cell surface receptor Nucleolin (NCL). To unravel how this apoptosis mechanism is induced in ECs, we performed RNA-seq using TS5-p45-treated ECs. Transcriptional changes under TS5-p45 treatment was further evaluated to highlight some of the caspase-dependent apoptosis-inducing and -suppressing genes which are altered in favor of apoptosis. Overall design: Total RNA samples from (12 h) TS5-p45-treated ECs and control ECs, in triplicates

本研究证实，TS5-p45可通过其细胞表面受体核仁素（Nucleolin，NCL）以半胱天冬酶（caspase）依赖的方式诱导内皮细胞（endothelial cell，EC）凋亡。为阐明该因子诱导内皮细胞凋亡的具体机制，本研究对经TS5-p45处理的内皮细胞开展了RNA测序（RNA-seq）。随后，本研究进一步评估了TS5-p45处理后的转录组变化，以筛选出一批受半胱天冬酶依赖调控的凋亡诱导基因与凋亡抑制基因，这些基因的表达改变均倾向于促进细胞凋亡。整体实验设计：收集经TS5-p45处理12小时的内皮细胞总RNA样本，以及未经处理的对照内皮细胞总RNA样本，每组均设置三次生物学重复。