Microdissected tumor epithelium and tumor stroma from patients with breast cancer

Stratification of breast cancers into subtypes are generally based on immune assays on tumor cells and/or mRNA expression of tumor cell enriched tissues. Here, we have laser microdissected tumor epithelium and tumor stroma from 24 breast cancer biopsies (12 luminal-like and 12 basal-like). We hypothesized that the stromal proteome would separate patients with breast into groups independently of the traditional epithelial based subtypes.

乳腺癌亚型的分层通常基于肿瘤细胞的免疫检测（immune assays），或富集肿瘤细胞的组织的mRNA表达谱。本研究对24例乳腺癌活检样本（含12例腔面样（luminal-like）乳腺癌与12例基底样（basal-like）乳腺癌）的肿瘤上皮与肿瘤间质实施了激光显微切割（laser microdissection）。我们提出如下假说：间质蛋白质组可将乳腺癌患者划分为不同组别，且该分类独立于传统的基于上皮的亚型分型体系。