Strome WA30534819_H3K4ME3_N2_L2_s4

modENCODE_submission_3549 This submission comes from a modENCODE project of Jason Lieb. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents. We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: N2; Developmental Stage: L2 Larva; Genotype: wild type; Sex: mixed Male and Hermaphrodite population; NUMBER OF REPLICATES: 1; EXPERIMENTAL FACTORS: Developmental Stage L2 Larva; temp (temperature) 20 degree celsius; Antibody WA305-34819 H3K4me3 (target is H3K4me3); Strain N2

modENCODE_submission_3549 本提交源自Jason Lieb主导的模式生物DNA元件百科全书（modENCODE）项目。如需查阅完整modENCODE项目列表，请访问http://www.genome.gov/26524648。项目目标：本分析聚焦于调控各类核心生物学元件的功能，包括确定核小体定位与占据状态、控制基因表达域、诱导X染色体基因沉默、介导有丝分裂分离与基因组复制、调控减数分裂过程中同源染色体配对与重组，以及维持细胞核内染色体空间定位的元件。本次研究共策略性筛选126个靶标，涵盖关键组蛋白修饰、组蛋白变体、RNA聚合酶II同工型、剂量补偿蛋白、着丝粒组分、同源染色体配对促进因子、重组标记物以及核膜组成成分。我们将整合本次实验产生的数据与现有靶标相关生物学知识，对缺失选定靶标蛋白的突变体及RNAi提取物开展染色质免疫沉淀芯片（ChIP-chip）分析，利用染色体外阵列技术评估候选序列基序在体内招募靶标的能力，鉴定选定靶标的组织特异性表达模式，并构建转录调控及全染色体功能的整合定量模型。关于数据使用条款与条件，请参阅http://www.genome.gov/27528022及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。实验类型：ChIP-chip。生物来源：菌株：N2；发育阶段：L2幼虫；基因型：野生型；性别：混合雌雄群体（雄性与雌雄同体）；生物学重复数：1次。实验因素：发育阶段为L2幼虫；培养温度：20摄氏度；抗体：WA305-34819，靶标为H3K4me3；菌株：N2