Gene expression signature and the prediction of liver metastasis in colorectal cancer by DNA microarray

Samples were taken from colorectal cancers in surgically resected specimens in 189 colorectal cancer patients. The expression profiles were determined using Affymetrix Human Genome U133 Plus 2.0 arrays. Comparison between the sample groups allow to identify a set of discriminating genes that can be used for molecular markers for predicting liver metastasis. Keywords: repeat One hundred and eighty nine patients who underwent surgical resection of colorectal cancer were included in this study. Patients were evaluated at 3-month intervals for the first postoperative year and at 6-month intervals thereafter. Liver metastases were identified by clinical and laboratory examination, completed by liver ultrasound or CT scan. Patients were divided randomly into a training and a validation set. A training set consisted of 160 patients, while a test set 29 patients.

本研究从189例结直肠癌患者的手术切除标本中获取结直肠癌样本。采用Affymetrix Human Genome U133 Plus 2.0基因芯片（Affymetrix Human Genome U133 Plus 2.0 arrays）测定样本的基因表达谱。通过不同样本组间的比较，可筛选出一组鉴别性基因，用作预测肝转移的分子标志物。
关键词：重复（repeat）
本研究纳入189例行结直肠癌手术切除的患者。患者于术后第一年每3个月随访一次，此后每6个月随访一次。通过临床与实验室检查，并辅以肝脏超声或CT扫描，确认肝转移发生情况。将所有患者随机划分为训练集与验证集：其中训练集包含160例患者，测试集包含29例患者。