Young and old HSCs from WT and Lnk-/- mice. Mus musculus

The adaptor protein Lnk is an important negative regulator of HSC homeostasis and self-renewal. This study aims to investigate the role of Lnk in HSC aging. Here we performed expression profiling of bone marrow CD150+CD48-LSK LT-HSCs from young and old WT and Lnk-/- mice. Results identify select Lnk-mediated pathways with potential involvement in HSC self-renewal and aging. Overall design: CD150+CD48-LSK HSCs were double sorted from WT and Lnk-/- mice at both young and old ages (2 months and 20 months, respectively). RNA was isolated using miRNeasy kit from QIAGEN and processed using the NuGEN Pico kit. The microarray analysis was performed at the Penn Molecular Profiling/Genomics Facility using GeneChip Mouse Gene 1.0ST array (Affymetrix).

衔接蛋白Lnk是调控造血干细胞（Hematopoietic Stem Cell, HSC）稳态与自我更新的关键负调控因子。本研究旨在探究Lnk在造血干细胞衰老过程中的作用。本研究对年轻与老年野生型（Wild Type, WT）及Lnk基因敲除（Lnk-/-）小鼠的骨髓CD150阳性CD48阴性LSK长期造血干细胞（Long-term Hematopoietic Stem Cell, LT-HSC）开展了表达谱分析。研究结果筛选出若干可能参与造血干细胞自我更新与衰老的Lnk介导调控通路。实验设计概述：分别从2月龄（年轻组）与20月龄（老年组）的野生型及Lnk-/-小鼠中双重分选得到CD150+CD48-LSK造血干细胞。采用QIAGEN公司的miRNeasy试剂盒分离RNA，并通过NuGEN Pico试剂盒完成样本处理。微阵列分析于宾夕法尼亚大学分子谱分析/基因组学设施完成，使用Affymetrix公司的GeneChip小鼠基因1.0ST芯片进行检测。