Candida albicans water soluble fractions (CAWS)-induced inflammation response in NOD1-deficient bone marrow derived dendritic cells

Candida albicans water-soluble fraction (CAWS)-induced coronary inflammation increased in NOD1-deficient mice. To investigate the molecular mechanisms underlying the aggravation of inflammation, gene expression in CAWS-stimulated bone marrow-derived dendritic cells (BMDCs) was evaluated. Overall design: Bone marrow-derived dendritic cells (BMDCs) derived from WT (C57BL/6) and Homozygous NOD1-KO mice were stimulated with 100 µg/mL CAWS. The cells were collected before CAWS stimulation and at 48 h and day8 after stimulation.

白色念珠菌水溶性组分（Candida albicans water-soluble fraction, CAWS）诱导的冠状动脉炎症在NOD1缺陷型小鼠（NOD1-deficient mice）中显著升高。为探究该炎症加剧的潜在分子机制，本研究对CAWS刺激的骨髓来源树突状细胞（bone marrow-derived dendritic cells, BMDCs）的基因表达水平进行了检测。实验设计：以野生型（WT，C57BL/6）及纯合NOD1敲除小鼠的骨髓来源树突状细胞为研究对象，采用100微克/毫升的CAWS进行刺激。分别于CAWS刺激前、刺激后48小时及刺激后第8天收集细胞。