Relative expression of the rhopalial opsins based on quantitative PCR.

Quantitative PCR ascertain the relative expression of Tc-leo and Tc-neo. In every run of the qPCR Tc-leo expressed relatively higher than Tc-neo. In every run the cycle threshold (CT) was lower for Tc-leo. In four runs Tc-neo was not sufficiently expressed to return a CT ascertaining the much higher relative expression of Tc-leo.

实时定量聚合酶链式反应（quantitative PCR，下称qPCR）用于测定Tc-leo与Tc-neo的相对表达量。在每一轮qPCR实验中，Tc-leo的相对表达量均高于Tc-neo，且其循环阈值（Cycle Threshold，CT）均更低。另有4轮实验中，Tc-neo的表达量不足以获得可检测的CT值，这进一步证实了Tc-leo相对表达量显著更高。