modENCODE_White Lab: genome-wide ChIP data of anti-Bonus-[GP37] from E16-24h on Illumina Genome Analyzer.

This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains genome-wide binding profile of the factor anti-Bonus-[GP37] from E16-24h generated by ChIP and analyzed on Illumina Genome Analyzer. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf A validated dataset is comprised of three biological replicates for ChIP-chip experiments and two replicates for ChIP-seq and meet the modENCODE quality standards. The control sample is the chromatin Input used for ChIP. Most factors binding profiles are generated by using specific antibodies for the protein of interest. However, some factors have been tagged by GFP in a transgenic line. In that case, ChIP is generated using an anti-GFP antibody. This submission represents the ChIP-seq component of the study

本数据集由芝加哥大学Kevin P. White牵头的果蝇调控元件modENCODE计划（modENCODE Project）所生成。其包含E16-24h阶段样本中抗Bonus-[GP37]因子的全基因组结合谱，该数据通过染色质免疫沉淀（ChIP）技术获取，并在Illumina基因组分析仪（Illumina Genome Analyzer）上完成分析。关于数据使用条款与细则，请参阅http://www.genome.gov/27528022 与http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。经验证的数据集包含3组生物学重复的染色质免疫沉淀芯片（ChIP-chip）实验数据，以及2组染色质免疫沉淀测序（ChIP-seq）实验重复，且符合modENCODE计划的质量标准。对照样本为染色质免疫沉淀实验所用的染色质输入对照。多数因子的结合谱通过针对目标蛋白的特异性抗体获取；然而，部分因子已在转基因品系中被GFP标记，此类样本的染色质免疫沉淀实验采用抗GFP抗体完成。本次提交为本研究的ChIP-seq相关实验组分。